133 – Essential Vitamins 2 + Folate ⭐ ❄

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Essential Vitamins 2 + Folate panel includes Vitamin D (25OHD), Cobalamin (Vitamin B12), Magnesium (Mg) three essential nutrients that are only obtained through diet, and Folate (Fol) which is produced endogenously through the gut bacteria as well as Total Calcium (tCa).  Vitamin D, the most important hormone in the body, is responsible for supporting the innate immune system and has a role in disease prevention and management.  Sufficient levels are anti-inflammatory, anti-proliferative, and pro-immunity. Cobalamin and Folate are chiefly response for DNA/RNA production as critical cofactors.  Magnesium is a cofactor in hundreds of enzymatic processes, and when low those reactions are less efficient.  Learn more.   VDI provides patient-specific dosing guidelines for both Vitamin D, B12, and Magnesium.

Clinical Applications:

  1. Diet Changes
  2. Gastrointestinal Disease
  3. Inflammatory Disease
  4. Cancer Patients
  5. General Wellness – preventative
  6. PLE Patients
  7. Patients on NSAIDs or Corticosteroids


Evaluating essential vitamin levels should start once the dog or cat reaches adult stages and establishes their adult diet.  Once diet has been stable for 3 months, test baseline vitamins and correct any insufficiencies with supplementation, or consider a diet change if values are considerably low.  VDI provides patient-specific dosing guidelines for Vitamin D,  B12 and Magnesium.  Recheck intervals are every year once sufficient unless diet or health changes occur.


Additional information


Canine, Feline

Patient Status

Acutely Ill, Apparently Healthy, Suspected Cancer, Suspected GI Disease


Folate (Fol), Magnesium (Mg), Total Calcium (tCa), Vitamin B12 (B12), Vitamin D (VitD)

Specimen Type

Serum (Frozen)


Recommended: 8hr